The lesbian, gay, bisexual, transgender, and queer/questioning (LGBTQ) Adolescent Health Study is a research initiative that engages a diverse range of stakeholders, both adolescent and adult, on the topic of LGBTQ adolescent health in Louisville, Kentucky. It is the first phase of a project to improve LGBTQ adolescent health outcomes, policies, and practices. Using a mixed methods approach, this phase explores the health issues that affect LGBTQ young people in Louisville as well as the barriers to, and facilitators of, good LGBTQ adolescent health in Louisville. Research activities consist of surveys and focus groups with adolescents and adult stakeholders. Surveys and focus groups with LGBTQ youths explore diverse topics pertaining to health and social experiences while qualitative interviews with adult stakeholders examine their health beliefs, experiences, knowledge, and local priorities as it relates to LGBTQ adolescent health in Louisville.